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Molecular diagnostic pcr handbook download -
This content was uploaded by our users and we assume good faith they have the permission to share /3233.txt book. If you own the copyright to this book and it molecular diagnostic pcr handbook download wrongfully on our website, we offer a simple DMCA procedure to remove your content from our site. Start by pressing the button below! Author: Gerrit J. Viljoen Louis H. Nel John R. Catalogue record for handbbook book is available from the Library of Congress. Printed in the Netherlands.
Aims of this адрес What is PCR? PCR principles What is the use of PCR? PCR and infectious diseases-the veterinary picture Laboratory Diagnostic Technology PCR for detecting Lumpy skin disease virus Other PCR assays Downlosd reactions Temperature moleculxr for moleculat PCR cycles Template DNA Primer concentration Example Example 3.
Design of primers Additional information Primer annealing Primer extension Degenerate PCR primers Design and use guidelines Storage of primers Example primer formulation Buffer and components Magnesium concentration Больше на странице and enzyme concentration Polymerase fidelity Denaturation time and temperature The Plateau effect Optimisinng g downnload PCR application Taguchi Method for PCR optimisation Agarose gel electrophoresis protocol also in Appendix B Loading buffer Electrophoresis buffer Visualising the DNA Ethidium bromide Electrophoretic conditions Inspection of gels Interpretation of PCR results A нажмите сюда PCR test result A negative PCR test result Confirming the identity of the PCR amplicon Nested Diagnosyic Hybridisation: the Southern blot Restriction fragment molecular diagnostic pcr handbook download polymorphisms RFLP Restriction endonucleases: General information Critical parameters Purity of DNA RE buffer Other downloas Sequence analysis General PCR problems PCR artifacts Polymerase chain reaction molecular diagnostic pcr handbook download Contamination of PCR Avoiding PCR contamination Useful pointers to remember Control experiments Sources of contamination Before PCR After PCR Prevention and elimination of contamination Chemical methods and materials that combat PCR molecular diagnostic pcr handbook download Ultraviolet-mediated DNA crosslinking Amplicon Carry-Over Prevention PCR Troubleshooting Low product yield No product, reaction failure Operator failure Template issues Temperature errors Reverse transcriptase Initiation of the RT reaction - Primers Oligo dT Hexanucleotide d N Specific oligonucleotide primer The RT reaction Anchored PCR Touchdown PCR Solid phase PCR Multiplex PCR The principle of a multiplex PCR Selection criteria Quantitative PCR Traditional quantitative PCR Diagnosric PCR Fluorometric real-time quantitative PCR Hybridisation probe chemistry Hybridisation probes diagrammatic representation Taqman probe chemistry Melting probe chemistry The laboratory set-up
- Download the PCR and Isothermal Amplification Handbook | Thermo Fisher Scientific - FI
Account Options Anmelden. Meine Mediathek Hilfe Erweiterte Buchsuche. E-Book anzeigen. Springer Shop Amazon. Gerrit J. Viljoen , Louis H. Nel , John R. In particular, molecular diagnostic methods have rapidly evolved in the past twenty years, since the advent of the Polymerase Chain Reaction PCR.
They are used in a wide range of agricultural areas such as, improving soil and water management; producing better crop varieties; diagnosing plant and animal diseases; controlling insect pests and improving food quality and safety. The uses of nucleic acid-directed methods have increased significantly in the past five years and have made important contributions to disease control country programmes for improving national and international trade.
These developments include the more routine use of PCR as a diagnostic tool in veterinary diagnostic laboratories. However, there are many problems associated with the transfer and particularly, the application of this technology.
These include lack of consideration of: the establishment of quality-assured procedures, the required set-up of the laboratory and the proper training of staff. This can lead to a situation where results are not assured. This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment.
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Molecular diagnostic pcr handbook download
This content was uploaded by our users and we assume good faith they have the permission to share this book. If you own the copyright to this book and it is wrongfully on our website, we offer a simple DMCA procedure to remove your content from our site.
Start by pressing the button below! Author: Gerrit J. Viljoen Louis H. Nel John R. Catalogue record for this book is available from the Library of Congress.
Printed in the Netherlands. Aims of this book What is PCR? PCR principles What is the use of PCR? PCR and infectious diseases-the veterinary picture Laboratory Diagnostic Technology PCR for detecting Lumpy skin disease virus Other PCR assays Basic reactions Temperature profile for the PCR cycles Template DNA Primer concentration Example Example 3. Design of primers Additional information Primer annealing Primer extension Degenerate PCR primers Design and use guidelines Storage of primers Example primer formulation Buffer and components Magnesium concentration Enzymes and enzyme concentration Polymerase fidelity Denaturation time and temperature The Plateau effect Optimisinng g a PCR application Taguchi Method for PCR optimisation Agarose gel electrophoresis protocol also in Appendix B Loading buffer Electrophoresis buffer Visualising the DNA Ethidium bromide Electrophoretic conditions Inspection of gels Interpretation of PCR results A positive PCR test result A negative PCR test result Confirming the identity of the PCR amplicon Nested PCR Hybridisation: the Southern blot Restriction fragment length polymorphisms RFLP Restriction endonucleases: General information Critical parameters Purity of DNA RE buffer Other factors Sequence analysis General PCR problems PCR artifacts Polymerase chain reaction inhibition Contamination of PCR Avoiding PCR contamination Useful pointers to remember Control experiments Sources of contamination Before PCR After PCR Prevention and elimination of contamination Chemical methods and materials that combat PCR contamination Ultraviolet-mediated DNA crosslinking Amplicon Carry-Over Prevention PCR Troubleshooting Low product yield No product, reaction failure Operator failure Template issues Temperature errors Reverse transcriptase Initiation of the RT reaction - Primers Oligo dT Hexanucleotide d N Specific oligonucleotide primer The RT reaction Anchored PCR Touchdown PCR Solid phase PCR Multiplex PCR The principle of a multiplex PCR Selection criteria Quantitative PCR Traditional quantitative PCR Competitive PCR Fluorometric real-time quantitative PCR Hybridisation probe chemistry Hybridisation probes diagrammatic representation Taqman probe chemistry Melting probe chemistry The laboratory set-up
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